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Cell culture and treatments The intestinal endocrine L cell line STC-1 and islet cell line NIT-1 were cultured in RPMI 1640 medium supplemented with 5 mM glucose, 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin in 100 mm cell culture dishes at 37 C in a 5% CO 2 atmosphere
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A growing body of preclinical research continues to explore its mechanisms and applications, including emerging work on neuroprotective effects and potential roles in spinal cord injury recovery
The accumulation of lipid hydroperoxide serves as a key trigger for initiating ferroptosis
The T4-targeted TALENs RNA mixture was microinjected into zygotes to obtain T4 KO mice, as described below
All authors contributed to and have approved the final manuscript
