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Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C

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In cytokine-stimulated astrocytes, arginine deprivation activates the amino acid-sensing kinase GCN2, leading to phosphorylation of eukaryotic initiation factor 2 (eIF2) and suppression of iNOS protein synthesis, thereby attenuating NO production