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Tracking: Log the date, site used, and any observations (redness, soreness, lumps)

At the end point, cells were washed 3 times with saline and stained for 20 min at room temperature using a saline solution containing 0.2 mM l-glutamic acid -(4-methoxy--naphthylamide) (GMNA

Anaesthesia was induced by the application of 3 l min 1 of volatile isoflurane with pure oxygen in the induction chamber of a COMPAC5 (VetEquip) small animal anaesthesia unit

These compounds can interact with the amino acid residues of proteins, mainly histidine, cysteine, and lysine, to form carbonyl derivatives known as advanced lipoxidation end-products (ALEs) (169)

A Note on the Underlying Molecule GHK-Cu is the copper-bound form of glycyl-L-histidyl-L-lysine, a tripeptide first identified in human plasma in the early 1970s by Loren Pickart

In that case, you'd want to confirm your results and determine the root cause