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Around 5 10 6 cells per 100-mm-diameter dish were transfected with the ecotropic packaging plasmid pCL-Eco (4 g) together with one of the following retroviral constructs (4 g): pMXs-Klf4, pMXs-Sox2, pMXs-Oct4 or pMXs-cMyc (obtained from Addgene) using Fugene-6 transfection reagent (Roche) according to the manufacturers protocol
doi:10.4103/0253-7613.91876
PMID: 49 Pi-SunyerXAstrupAFujiokaKGreenwayFHalpernAKrempfMet al
Patients who meet the criteria for a GLP-1 are also enrolled in a clinically proven lifestyle modification program with behavior change support from an Omada Health care team, designed to help them achieve and maintain improved health outcomes whether that is controlled A1C, weight loss, or reducing cardiovascular comorbidities
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