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However, some individuals may experience mild gastrointestinal discomfort or allergic reactions

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acceptance criteria and obtained values are reported in Section "HPLC method validation" (as per ICH Guidelines): System Precision: 5 replicates, RSD 1% Linearity and Range: r2 0.99 Method Precision: 5 replicates, RSD 1% Selectivity: no interference in control tissue Accuracy and Recovery: spiking and comparison with known standards LOD and LOQ: 3:1 and 10:1 signal/noise ratios Ruggedness & Robustness: RSD 6% under varying conditions LOD and LOQ were determined using signal-to-noise ratios of 3 and 10, respectively