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Specificity of staining was confirmed by staining with a rat IgG (6-001-F, R&D Systems, Bio-Techne), a Rabbit IgG (ab27478, Abcam) or a mouse IgG1, kappa (Abcam, ab18443) isotype controls
10 mg in 1 mL floor of total dose 20 units ( 0.2 mL ) on a 0.3 mL syringe for a 2000 mcg dose
Conservative approach: Stick with well-researched peptides (BPC-157, TB-500, established growth hormone peptides) rather than experimental options with minimal evidence
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