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Polymerase chain reaction (PCR), library preparation, and 16 S rRNA gene sequencing: The V3-V4 hypervariable region of the 16 S rRNA gene was amplified using the primer pair ((Forward = 5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3) and (Reverse = 5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3))
But the arrival of GLP-1 (glucagon-like peptide-1) receptor agonists changed everything
A gastric sleeve is a *restrictive* procedure (making the stomach smaller) but also a *metabolic* one, as it cuts away the part of the stomach that produces the hunger hormone
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As a result, patients not only achieve better glycemic control but also experience healthy weight loss
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