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A small amount of the ingested vitamin B12 (one to two per cent) is absorbed by passive diffusion simply crossing the cell membranes without binding to anything (Temova Rakua et al., 2022)

Weight on the scale might drop 2-4 pounds (depending on starting point and deficit)

It is also obtained through dietary sources, primarily meat and fish

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This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS