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Measurement of the levels of intracellular GSH and the relative expression of NRF2, GPX4 and SOD1 in tissue samples following neoadjuvant chemotherapy To further verify the role of GSH metabolism in chemotherapy resistance, we analyzed breast cancer data from the Gene Expression Omnibus (GEO) dataset (GEO: GSE140494)

For NADP + and NADPH assays, 20 L (for NADP + assay) or 30 L (for NADPH assay) of the neutralized sample extract was combined with 90 L of assay mix consisting of 50 L of 0.1 M HEPES containing 2 mM EDTA (pH 7.5), 20 L of 1.2 mM 2,6-dichlorophenolindophenol, 10 L of 20 mM phenazine methosulfate, and 10 L of 10 mM glucose-6-phosphate

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