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When GSH is administered as therapeutic agent, its metabolism or effect can also measured by glutathione assay.
The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 M FOXO4-DRI was added in the medium immediately
For example, EA was conjugated to 2-amino-2-deoxy-d-glucose (EAG) 90 , obtaining an adduct with significant anticancer activity such as EA but without the undesired diuretic activity 90
It also raises luteinizing hormone (LH) in ovariectomized rats, but doesnt affect FSH
Huttunen, K