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On the day of assay 2 g per well of the cell membrane was incubated with furimazine (1:1000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)
J Physiol.
Individual experiences may vary
The peak incidence was estimated to be 180,000 cases per year in the mid-1980s, but the rate declined to approximately 19,000 new cases by 2006
That cleared the runway
Whether any of the symptoms described above represent such a conversion is unknown
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