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After overnight culture, the cells were co-transfected with pGloSensor22F cAMP plasmid (Promega) and GLP-1R DNA using Lipofectamine 2000 transfection reagent (Invitrogen)

The outcome incident or recurrent diagnosis of CUD (International Classification of Diseases, Tenth Revision (ICD-10) code F12 Cannabis related disorders) that occurred within the 12-month time window after the index event) were compared between matched cohorts

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Additionally, it participates in bettering your immune system, tissue generation and development, enhances anticancer ability, maintains anti-ageing properties, and counterbalances free radicals