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Cells were resuspended in ice-cold PBS plus 0.1% BSA and processed using a BD LSR II flow cytometer (10,000 cells/sample) for receptor trafficking, or BD LSRFortessa X-20 for surface downregulation

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snRNA-seq libraries were prepared using the 10X Genomics Chromium Next GEM Single Cell 3 Reagent Kits v.3.1 with loading of ~12,000 total nuclei per sample and sequenced on the Illumina HiSeq 4000 instrument

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This enzyme plays an important role in methylation pathways that influence cellular metabolic signalling and intracellular energy regulation

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