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GSH is synthesized from cysteine, glutamate, and glycine by the ATP-dependent enzyme -glutamyl-cysteine (-GC) ligase (GCL) synthetase, which is composed of the -GC ligase catalytic subunit (GCLC), the -GC modifier subunit (GCLM), and GSH synthetase (GSS) 24
The constant levels of marker proteins such as GAPDH, combined with observations indicating that knockdown of the UPR element, BiP/Grp78 , revealed no detectable effects on Nrf2, GCLC, or glutathione, further support the conclusion that NFT-mediated stabilization of Nrf2 is a major contributor to its accumulation and the subsequent stimulation of GCL and glutathione synthesis
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After 1 min, stop solution was added to terminate the reaction
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