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The resulting buffy coat was collected, resuspended in PBS, and centrifuged again (200 x g, 8 min, 20C, Universal 320, Hettich, Tuttlingen, Germany)

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Missing doses or taking a lower dose than prescribed decreases the efficacy of these medications

A current or past history of eating disorders or disordered eating is another reason to use extra caution, because these medications can worsen or reignite those symptoms

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For Parkinsons disease, the study utilized a unilateral 6-OHDA lesioned rat model