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Activated PERK, phosphorylates eukaryotic initiation factor 2 (elF2) leading to cell cycle arrest and upregulates important transcription factors (ATF4, CHOP and Nrf2)
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Because of its high prevalence in conjunction with obesity, diabetes, and insulin resistance, NAFLD has been increasingly appreciated as a hepatic manifestation of metabolic syndrome and is the leading cause of death from liver disease in the United States 1,2,3
Labeled larvae were then rinsed once with PBS and stored in 1:1 PBS:glycerol for confocal imaging
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