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In Italy, the classes under consideration include GLP-1 receptor agonists (dulaglutide, exenatide, exenatide LAR, liraglutide, lixisenatide, semaglutide) and also GIP/GLP-1 dual agonists (tirzepatide)

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Recombinant adeno-associated serotype 9 viruses with Ap2 promoter for SIRT3 overexpression in adipocytes (AAV- Ap2 -SIRT3) or the empty vector (AAV- Ap2 ) were acquired from GeneChem Co, Ltd (Shanghai, China)