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Overall, the identification of these GO terms and KEGG pathways provides a framework for understanding the molecular mechanisms underlying the biological processes investigated in this study

Collectively, these studies support its experimental relevance in exploring GLP-1-mediated pathways and their potential to enhance metabolic and cardiovascular understanding in non-clinical settings

Risk factors that may influence the decision to pause treatment include: Being in the dose-escalation phase of GLP-1 therapy Experiencing active gastrointestinal symptoms (nausea, vomiting, bloating) Having known gastroparesis or other conditions affecting gastric emptying Taking concurrent medications that further delay gastric emptying (e.g., opioids) Undergoing procedures with higher aspiration risk (e.g., emergency surgery, airway surgery) If your anaesthetist or surgical team advises temporary cessation based on your individual risk assessment, the timing will depend on the specific medication: Daily injections (such as liraglutide): Typically paused 24 hours before surgery Weekly injections (such as semaglutide, dulaglutide): Often paused with the last dose given at least 7 days before surgery Oral semaglutide (Rybelsus): Usually paused 24 hours before surgery [4] However, these timings may vary according to local NHS Trust protocols and individual clinical assessment

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ISH, GLP-1R expression detected by transgene expression in mice [ Table 1

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