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Abstract Parkinsons disease (PD) is a neuromotor disorder, primarily manifested by motor anomalies due to progressive loss of dopaminergic neurons

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Complementary DNA was synthesized using 1 g of RNA, 1 l of oligo dT (500 g/ml), and 1 l of 10 mM dNTP mix in a 10 l reaction

The AUCell package (version 1.8.0) (19) utilizes a rank-based scoring approach to evaluate the activity levels of these pathways, generating a gene set activation score for each cell through the Area Under the Curve (AUC) function