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Furthermore, there is evidence to suggest that chronic SSRI use may be associated with the development of osteoporosis, with the inhibition of bone matrix deposition being a potential mechanism [88, 90]
The Western blot method referred to our previous study 49
Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model
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Mice exhibiting fasting blood glucose levels above 16.7 mmol L 1 were considered as successfully established and were subsequently randomly assigned into six groups (n = 6): PBS (Blank control group), G, M-CuP solution, D-CuP solution, G/M-CuP and G/D-CuP
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