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doi: 10.1016/j.phymed.2012.07.001

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Slowly warm the microplate with the reagents up to the temperature you will run the experiment (preferably in an incubator at 37 degrees C) Once the elution stage is over, take both microplates out of the incubator and transfer 15 microliters of each eluted sample to the corresponding well of the reagent microplate (already containing the reagents)

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