Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and incubated overnight with primary antibodies at dilutions specified in Supplementary Table 26
Higher concentration: mixing to 10 mg/mL keeps injection volumes small even at the top of the range useful when titrating toward 4.5 mg, the dose linked with the largest weight reductions in early trials [4]
Due to its central role in fatty acid transport and energy metabolism, L-carnitine is frequently examined in studies focused on metabolic processes, oxidative pathways, and mitochondrial function
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