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Biochemical Pharmacology, 2024, DOI: 10.1016/j.bcp.2024.116565,

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The mRNA levels for modulated genes were determined by reverse transcription of total RNA followed by quantitative real-time PCR analysis (qRT-PCR) on a Quant Studio 6 Flex system (Applied Biosystems, Life Technologies, U.S.) using SYBR PCR Master Mix reagent kits (Takara, Japan) following the manufacturers protocol

However, some people may need to do it more frequently if they have a particularly serious condition