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The optimal conditions were two mmol L 1 peroxide, 500 mol L 1 GSH, and 10 min incubation time
If youve used a high concentration of pH buffer, such as 50mM Tris pH 8, then the addition of 10-20mM glutathione may not significantly change the pH of your buffer
Therefore, the extent of heat stress-induced damage depends on temperature intensity as well as the duration and frequency of heat exposure and individual thermal adaptation capacity (Robinson et al., 2023)
median [IQR] age, 47 [32-59] years
Diabetes Care 33 , 17591765 (2010)
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