The cytotoxic and oxidative effects of test compounds on cultured cells can be determined using the CellTox Green Cytotoxicity Assay and the GSH/GSSG-Glo Assay
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In this study, HepG2 cells transiently transfected with the human GSTP1 construct were utilized to investigate the protective effect of GSTP1 in a cellular context
Despite no obvious mechanism illustrating MTX-induced organ dysfunction, accumulation of MTX polyglutamate inside hepatocytes, the metabolized form of MTX, has been reported as the key factor of MTX-induced hepatotoxicity
Oxidative stress in the CNS acts as a persistent inflammatory trigger by activating brain-resident immune cells, particularly microglia and astrocytes
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