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After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody

To explore through an alternative strategy whether GLP-1R binding kinetics influence PM recycling and insulin secretion, we utilized the GLP-1R allosteric modulator 4-(3-( b enzyloxy)phenyl)-2-( e thylsulfinyl)-6-( t rifluoro-methyl) p yrimidine (BETP), which alters the residence time of orthosteric GLP-1R agonists 45

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