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After drying of gel pieces in a vacuum concentrator, proteins were digested with 80 ng trypsin per gel band in 100 mM HEPES in water (p H 6.8) overnight at 37 C

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146 A meta-analysis of findings from seven randomized controlled trials involving 217 fibromyalgia patients found high-frequency (10-Hz) rTMS, typically performed five times per week for two to four weeks, effectively reduced pain and improved quality of life but did not relieve depression symptoms