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One hundred microliter aliquot of tissue homogenate supernatant was first incubated with 50 L KOH (1 mol/L) at 37 C for 30 min to fully hydrolyze the combined carnitine, and 10 L HCl (1 mol/L) was then added to neutralize the solution (sample A)

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54 patients showed intermediate to advanced fibrosis (stage F2-4) and 74 fibrosis stage F0-1

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