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2000s: Palatin Technologies modified MT-2's structure to create bremelanotide (PT-141), retaining MC3R/MC4R selectivity for sexual function while reducing MC1R activity (tanning) and improving the pharmacological profile
Blood 81(10):2702-7, 1993
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So, you would have already purified proteins corresponding to the full-length and each individual domain of the interacting protein, and now you do a pull-down to see which ones bind
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