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The aortas were then incubated in freshly prepared SA--Gal staining solution at pH 6.0 overnight at 37 C, according to the manufacturers protocol
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Potential Side Effects Possible side effects include allergic reactions, which might manifest as skin rashes, itching, or even more severe responses
A later review described DSIP as a still unresolved riddle, emphasizing that the sleep-factor hypothesis, receptor biology, and gene-level interpretation remain poorly documented [2]
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