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Cell sample Harvesting cell should not less than 106,then wash it with PBS for twice (re-suspend cell with PBS, centrifuge at 600 g for 10 minutes)
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As a positive control, we confirmed the ability of nimodipine to suppress the increase of [Ca 2+ ] i that resulted from 28 mM KCl-induced membrane depolarization (Fig
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doi: 10.1111/dom.14286 23 CarusoICignarelliAGiorginoF
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