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The reaction mixture consisted of 0.025 mM MgCl 2 , 200 mM pentoxyresorufin, 0.32 mg of liver microsomal proteins, and 130 mM NADPH in 2.0 mL of 0.05 M Tris-HCl buffer (pH 7.4)

Any T cells that escape this rigorous thymic negative selection are then targeted by regulatory T cells (Tregs), which act as a crucial secondary checkpoint (25, 26)

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Both are worth investigating, though for different reasons