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Pneumococci were cultured in Todd-Hewitt broth supplemented with 0.5% yeast extract (THY), chemically defined medium (CDM) or tryptic soy agar (TSA) plates with sheep blood (3%) at 37C, 5% CO 2 as previously described (Lu et al., 2006)

Relative ratios of the spot densities between BPC-157-treated and control samples were calculated