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Sample sections were blocked in 5 % goat serum with 0.3 % Triton X-100 for 1 h at RT and then incubated overnight at 4 C with the following primary antibodies: mouse anti-GLP-1R (1:50, Santa Cruz, CA, USA), mouse anti-GLP-1(1:50, Santa Cruz, CA, USA), rabbit anti-Iba1 (1:500, Wako Chemicals, Tokyo, Japan), rabbit anti-GFAP (1:200, Cell Signalling, Boston, MA, USA), rabbit anti-NeuN (1:100, Proteintech, China), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), and mouse anti-c-fos (1:100, Santa Cruz, CA, USA)
Third-party testing is preferred
[73] Al Alawi AM, Majoni SW, Falhammar H
Therefore, the heterogeneities in cardiovascular medication, baseline characteristics, study population, and study design should be taken into consideration when comparing the results among these trials
Strategies to use GDNF gene vectors have not been successfully so far ( Glucagon-like peptide 1 and glucose-dependent insulinotropic peptide are growth factors that can cross the bloodbrain barrier GLP-1 and GIP are peptide hormones and growth factors that play important physiological signaling roles to control cell metabolism and energy utilization (Zhang et al., 2020)
Ethics declarations Animal Ethics declaration The NIH's Guide for the Care and Use of Laboratory Animals was adhered to in every procedure
