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In general, the brain activity of AMPK is activated by fasting and is inhibited by re-feeding [88, 122, 123], but the effect of glucose on AMPK also regulates expression in VMH [134]

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It acts as a free radical scavenger, upregulating antioxidant enzymes such as superoxide dismutase and catalase (9)

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In vitro cAMP assay For GLP-1R agonist activity, CHOK1 cells stably expressing human GLP-1R were used to measure peptideantibody conjugate-induced cAMP production in a homogenous time-resolved fluorescence assay (Cisbio, cat

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DOI: 10.1371/journal.pone.0130189 58

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