Then, TMB chromogen solution (CWBIO) was added, and the absorbance at 450 nm was measured via a SpectraMax plate reader (Molecular Devices)
We further examined the protein expression and activation states of Smad2 and Smad3, which are downstream effectors of TGF signaling, in NP cells treated with L929 conditioned media
Full reconstitution guide Advanced calculator Dosing & Reconstitution Guide A single practical dilution with simple unit math, step by step Standard / Gradual Approach (3 mL = ~3.33 mg/mL) Reconstitute: Add 3.0 mL bacteriostatic water to one 10 mg vial final concentration ~3.33 mg/mL
Stock solutions were prepared and dissolved following manufacturers instructions
For injured tissue, enhanced blood supply means improved delivery of oxygen, nutrients, and repair cells to the damage site
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