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Pre-treatment of HGF with trisulfides and nitration Recombinant mouse HGF (carrier protein-free) was pre-treated with glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS) at mole ratios (HGF : trisulfide) of 1:0 (with solvent alone), 1:400, 1:1000, and 1:4000 at pH 7.4, 25C just prior to the peroxynitrite protocol (optimized at a molar ratio 1:2000 relative to HGF, pH 7.4, 37, for 30 min), which was previously demonstrated to induce maximal nitration of Y198 and Y250 located in the -chain under physiological conditions as described previously 32

If unusual symptoms such as eruption, pale face, blood pressure drop, or difficulty in breathing occur during therapy, discontinue the drug immediately

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10.1016/j.nut.2018.03.058 52 TryfidouD

PCR master mixes (SYBR Green dye and FastStart Taq DNA polymerase were supplied) were prepared according to the manufacturer's instructions

Effect of ischaemia-reperfusion on glutathione peroxidase, glutathione reductase and [PMID: 2318422] Laboratory literature summary: Effect of ischaemia-reperfusion on glutathione peroxidase, glutathione reductase and glutathione transferase activities in research