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Once the samples reached confluency the cells were subcultured and all tendon fibroblasts between passages 2 and 4 that had a proper growth rate and normal fibroblast shape were used in the experimentation process

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doi: 10.2337/dc21-S009 50 BaggioLLYustaBMulvihillEECaoXStreutkerCJButanyJet al

The samples were diluted by 100 times and measured using LCMS in the selective ion mode

Chen JJ, Thiyagarajah M, Song J, Chen C, Herrmann N, Gallagher D, et al

or: Hlscher C