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Replacement of Lys105 by uncharged residues in K105A/Y resulted in a 6597% decrease of the k cat app values for GSSCys and GSH (Fig

It is important to note that only the oxidized forms of NAD (NAD + and NADP + ) were included in the comparative validation due to instability of reduced forms (NADH and NADPH) in MS analyses

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The free radical scavenging activity of hydroxytyrosol, when consumed as part of EVOO, far exceeds that of isolated supplements, offering a more holistic, natural benefit for your body

We therefore sought a strategy to reduce the scale of the library to remain within the quantitative throughput limits of our assay (up to ~40,000 substrates), while still assaying a full repertoire of sequences likely to be cleaved by our evolved proteases (Extended Data Fig