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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

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The red blood cells job is to transport oxygen to many other parts of the body

### Discard the Vial If: - It has been more than 30 days since reconstitution - You see cloudiness, particles, or color changes - The vial was left at room temperature for more than a few hours - You suspect contamination (e.g., the stopper was touched without cleaning) When in doubt, discard and start fresh

On iron metabolism and its regulation