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USA 104 , 1530515310 (2007)
Meng C, Zhan J, Chen D, Shao G, Zhang H, Gu W, et al
By adding a mixture of reduced and oxidized glutathione, your protein can sample different disulfide bond states and should end up in the correct native state, if that is the most energetically favorable (Figure 6)
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PMID: 36178333
Methods Cell lines Spodoptera frugiperda 9 ( Sf 9) (Invitrogen) and High Five insect cells (Expression Systems) were cultured in ESF 921 serum-free medium (Expression Systems) at 27 C and 120 rpm
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