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Why cysteine is the rate-limiting factor: The intracellular concentration of cysteine sits close to the Km value of GCL (0.1 to 0.3 mM), meaning the enzyme is operating near its saturation point under normal conditions
Furthermore, several inhibitors (e.g., DC-TEADin02 and K-975) that target the autopalmitoylation pocket of the TEA domain (TEAD) of transcription factors occupy this hydrophobic cavity, where they competitively inhibit TEAD autopalmitoylation and consequently disrupt its oncogenic transcriptional activity [187, 188]
Nadolski, M
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When collagen is broken down (for instance during injury), GHK is released and can bind copper