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Additionally, compared to the comparable groups, rats treated with oil extract for 14 days showed greater recovery, with TNF- concentrations dropping to 15.37, 12.78, and 21.62 pg/ml for groups 5, 6, and 7, respectively (2, 3, and 4)
The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM
PMCID: PMC10148537
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