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We used a human BBB co-culture model improved by cARLA treatment, as described in our paper [40], for the impedance, cellular uptake and permeability experiments

Not always required for standalone therapy, but we strongly recommend it

BPC-157 + TB-500 is a multi-component laboratory reference blend supplied for qualified research and analytical applications only

This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS