Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI
Thats HALF PRICE
35 mm dishes were then transferred to a luminometer (Lumicycle32, Actimetrics) and 24-well plates to a luminometer/incubator (CL24A-LIC, Churitsu Electric Corp.)
If the skin lacks the elasticity to contract over a newly reduced area, the result can appear hollow or sagged rather than contoured
A doctor will inject the shot into the muscle or deep into the skin
The formulation (pure peptide, proprietary blend, lyophilized vs solution) matters