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They also determined the rate of dissociation of insulin using advanced sampling techniques with two CVs: the separation between the center-of-mass of each monomer and the number of cross contacts between the C atoms of each monomer

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Njalsson, R., Ristoff, E., Carlsson, K., Winkler, A., Larsson, A

Human data is still limited, so individual results vary which is exactly why this is done with monitoring rather than guesswork

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It represents a scientifically determined window where, assuming proper refrigerated storage and sterile technique, the benzyl alcohol can reliably maintain the sterility of the solution despite multiple punctures of the stopper