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Gastric nucleotide-binding domain, leucine-rich repeat, and pyrin domain PYD containing protein 3 (NLRP3) and gasdermin D (GSDMD) mRNA expression levels were estimated by quantitative real-time PCR

In spinal cord tissues from patients with ALS, the expression of arachidonate 5-Lipoxygenase (ALOX5) and complement component 3 (C3) was markedly upregulated, accompanied by reduced GSH levels, elevated iron concentrations, and enhanced lipid peroxidation

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10.1089/ars.2005.7.919 Antioxid

Oxygen consumption rate (OCR) was measured using a Seahorse XFe24 Analyzer (Agilent Technologies) under basal conditions and following sequential injection of oligomycin, FCCP, and rotenone/antimycin A

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