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The continuous use approach represents traditional treatment strategy where daily GHK-Cu application continues indefinitely or until goals achieved, then potentially reduces to maintenance frequency (every other day or few times weekly) rather than complete discontinuation

The bedtime timing matters because the goal is to amplify a pulse that would otherwise occur naturally during deep sleep, not to introduce a daytime pulse that conflicts with normal pituitary rhythms

Samson RR, McClelland DB

(Wounds, 2003) documented GHK-Cu's effects in animal wound models, while additional studies have examined its role in bone remodeling, nerve tissue research, and hair follicle biology

The peptide enhances fat oxidation while preserving lean muscle mass, making it valuable for body recomposition goals

After single IV administration, the t 1/2 and AUC 0t of BPC157 in dogs were 5.27 min and 76.4 30.2 ng min/ml