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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

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CT scan shows diffuse low-density in subcortical areas, suggesting cerebral edema

We also found genetically predicted higher acetyl-carnitine was associated with lower HDL-cholesterol in men and possibly also in women (Additional file 2: Fig

The genetic variable underneath Methylation and B12 metabolism Variants in MTHFR, MTRR, and COMT shape the baseline methylation and B12 metabolism that MIC-B12 acts on

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