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Heat your liquid (never boil)
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A gradient of eluents A (distilled water with 0.1% v/v formic acid, 85171, Thermo Fisher Scientific) and B (acetonitrile with 0.1% v/v formic acid, 85175, Thermo Fisher Scientific) was used to achieve separation (300 nl/min flow rate), from 2% B to 40% B in 88 minutes
Using both together produces a much bigger GH pulse than either one alone
The end result is that less glucose is able to move into the muscle cell and be converted into glycogen
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